merck millipore,默克密理博,FTAV00003,Fast Trap Adenovirus Purification and Concentration Kit

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merck millipore,默克密理博,FTAV00003,Fast Trap Adenovirus Purification and Concentration Kit
产品名称：Fast Trap Adenovirus Purification and Concentration Kit
产品型号：FTAV00003

merck millipore,默克密理博,FTAV00003,Fast Trap Adenovirus Purification and Concentration Kit

产品介绍

merck millipore,默克密理博,FTAV00003,Fast Trap Adenovirus Purification and Concentration Kit

描述
产品目录编号	FTAV00003
商名	Fast-Trap
描述	Fast Trap Adenovirus Purification and Concentration Kit
概述	The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x10¹³ total adenovirus particles. The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit. For laboratory use only; not for diagnostic or in vivo use.

产品信息
部件	3 – Fast-Trap virus filter units 3 – Steriflip-HV filter units 1 – 10x Binding buffer, 20 mL 1 – Equilibration buffer, 100 mL 1 – Wash buffer, 200 mL 1 – Elution buffer, 21 mL 3 – Amicon Ultra-4 filter units, 50 kDa NMWL 1 – Test tube stand 1 – User Guide 1 – Overview of Procedure card 1 – Material Safety Data Sheet

应用
主要应用	Adenovirus Purification and Concentration

产品使用声明
使用声明	General Lab Use

储存和货运信息
存储条件	The Fast-Trap Adenovirus Purification and Concentration Kit should be stored at room temperature. Warnings and Precautions: WARNING: This kit permits quick purification of adenovirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit. WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet. For research use only. Not for use in diagnostic or human clinical procedures. Variation in the amount of adenovirus purified with this kit may be due in part to infection success and cell conditions.

储存和货运信息

存储条件

The Fast-Trap Adenovirus Purification and Concentration Kit should be stored at room temperature.

Warnings and Precautions:
WARNING: This kit permits quick purification of adenovirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective
equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit.

WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet.
For research use only. Not for use in diagnostic or human clinical procedures.
Variation in the amount of adenovirus purified with this kit may be due in part to infection success and cell conditions.