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merck millipore,默克密理博,MAB13406,Anti-MMP-2 Antibody, pro and active form, clone VB3
产品名称：Anti-MMP-2 Antibody, pro and active form, clone VB3
产品型号：MAB13406
This Anti-MMP-2 Antibody, pro & active form, clone VB3 is validated for use in ELISA, IF, WB for the detection of MMP-2.
merck millipore,默克密理博,MAB13406,Anti-MMP-2 Antibody, pro and active form, clone VB3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB13406 Replaces 04-1048 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-2 Antibody, pro and active form, clone VB3 Alternate Names - 72 kDa Type IV Collagenase
- Gelatinase A
产品信息 格式 Purified 控制 - POSITIVE CONTROL: Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta. Bladder, breast and ovarian carcinomas.
演示 200 μg/mL. of antibody purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 This Anti-MMP-2 Antibody, pro & active form, clone VB3 is validated for use in ELISA, IF, WB for the detection of MMP-2. 主要应用 - Western Blotting
- ELISA
不建议的应用 - Immunohistochemistry
应用说明 Western blot: 1:200-1:400 for 2 hours at room temperature
ELISA
Immunofluorescence
Does not work for immunohistochemistry Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human native 72 kDa Gelatinase A. 表位 pro and active form 克隆 VB3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody recognizes proteins of 72kDa and 66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72 kDa collagenase IV or gelatinase A). Shows no cross-reactivity with pro and active forms of other MMPs. MMPs are proteolytic enzymes capable of degrading connective tissue components. Degradation of the extracellular matrix (ECM) is an essential step in tumor invasion and metastasis. MMPs each have different substrate specificities within the ECM and are important in its degradation. MMP-2 mainly degrades type IV collagen and denatured collagens. MMP activity is modulated by tissue inhibitors of metalloproteinases (TIMP). Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by TIMP have been implicated in the invasive potential of malignant tumors.Cellular Localization: cytoplasmic 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004530.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. 基因符号 - EC 3.4.24.24
- MMP2
- TBE-1
- CLG4A
- CLG4
- MMP-II
- MONA
- MMP-2
UniProt编号 - P08253
UniProt汇总 FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 660 amino acids; 73882 Da
SUBUNIT: Ligand for integrin alpha-V/beta-3.
TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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