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merck millipore,默克密理博,FCMAB110P,Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate
产品名称：Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate
产品型号：FCMAB110P
Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC.
merck millipore,默克密理博,FCMAB110P,Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC M Phycoerythrin Monoclonal Antibody 描述 产品目录编号 FCMAB110P 描述 Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate 长描述 Ataxia Telangiectasia Mutated Kinase Alternate Names - A-T, mutated <br />AT mutated <br />TEL1, telomere maintenance 1, homolog <br />ataxia telangiectasia mutated <br />ataxia telangiectasia mutated (includes complementation groups A, C and D) <br />ataxia telangiectasia mutated protein <br />human phosphatidylinositol 3-kinase homolog <br />serine-protein kinase ATM
背景信息 Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981. 产品信息 格式 Phycoerythrin 控制 - Irradiated HeLa cells
演示 Purified mouse monoclonal IgG1κ conjugated to phycoerythrin in PBS with less than 0.09% sodium azide and 15 mg/mL BSA. 应用 应用 Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC. 主要应用 - Flow Cytometry
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to human ATM phosphorylated at Ser1981. 表位 Phosphorylated at and around Ser1981 克隆 10H11.E12 宿主 Mouse 特异性 Antibody recognizes ATM phosphorylated at Ser1981. 同种型 IgG1κ 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse. Predicted to cross-react with rat based on sequence homology 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000042
Entrez基因汇总 The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - TPLL
- ATM
- AT
- TELO1
- ATC
- DKFZp781A0353
- MGC74674
- ATD
- ATA
- AT1
- T-PLL
- ATE
- TEL1
- ATDC
修改 - Phosphorylation
纯化方法 Protein G purfied UniProt编号 - Q13315
UniProt汇总 FUNCTION: SwissProt: Q13315 # Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function.
SIZE: 3056 amino acids; 350644 Da
SUBUNIT: Exists in monomeric and tetrameric state. Binds DNA ends, p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. Interacts with EEF1E1. This interaction, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. Interacts with DCLRE1C. Interacts with MYST1. Interacts with HTATIP.
SUBCELLULAR LOCATION: Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
TISSUE SPECIFICITY: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.DOMAIN:SwissProt: Q13315 The FATC domain is required for interaction with HTATIP.
PTM: Phosphorylated by ARK5. Autophosphorylated on Ser-1981 upon DNA damage. & Acetylated by HTATIP upon DNA damage; which is required for autophosphorylation and subsequent activation.
DISEASE: SwissProt: Q13315 # Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation. & Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients. & Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL). & Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 1 PI3K/PI4K domain.产品使用声明 质量保证 Evaluated by Flow Cytometry with HeLa cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light. 包装信息 数量 100 tests
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