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merck millipore,默克密理博,FCCH100107,FlowCellect MitoLive Kit
产品名称：FlowCellect MitoLive Kit
产品型号：FCCH100107
This FlowCellect MitoLive Kit for flow cytometry includes MitoSense Red, a fluorescent cationic dye & Calcein acetoxymethylester, a non-fluorescent, cell permeant compound & provides a measure of cell More>>
merck millipore,默克密理博,FCCH100107,FlowCellect MitoLive Kit
- 产品介绍
描述 产品目录编号 FCCH100107 商名 - FlowCellect
描述 FlowCellect MitoLive Kit 概述 Millipore’s FlowCellect™ MitoLive Kit includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria and is responsive to mitochondrial potential changes and Calcein acetoxymethylester (calcein-AM) a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein and provides a measure of cellular vitality. The simultaneous use of the reagents allows researchers to obtain information on early and late apoptosis in one simple assay. MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 channel fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. The cell permeant dye, Calcein-AM included in the kit, enters all cells and if the cell is vital is hydrolyzed by intracellular esterases into the fluorescent anion calcein which are retained in cells with intact membranes. Healthy cells thus exhibit high green fluorescence. Cells in which the intracellular esterases are impacted on treatment or have compromised membranes demonstrate low green fluorescence. The assay kit allows the identification of 3 populations of cells; healthy live cells with intact membrane potential, live cells with impaired mitochondria and non-vital or dead cells with impaired mitochondria. The entire assay can be performed in approximately 30 min once cellular samples are ready. Alternate Names - FlowCellect
产品信息 部件 - User Manual
- MitoSense Red Dye
- Calcien-AM
- 10X Assay Buffer
检测方法 Fluorescent 应用 应用 This FlowCellect MitoLive Kit for flow cytometry includes MitoSense Red, a fluorescent cationic dye & Calcein acetoxymethylester, a non-fluorescent, cell permeant compound & provides a measure of cellular vitality. 主要应用 - Flow Cytometry
生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 100 Tests
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