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重要规格表
宿主 M 描述 概述 Recognizes the ~88 kDa wild-type EBNA from EBV-transformed human cells as well as the ~31.5 kDa mutant form of EBNA in Raji cells. 产品目录编号 DP15L 品牌系列 Calbiochem®
同义词 Anti-Epstein Barr Nuclear Antigen 参考 参考 Freese, U.K., et al. 1983. J. Virol. 48, 731.
Fischer, D.K., et al. 1981. Cell 24, 543.
Orellana, T., et al. 1977. J. Virol. 22, 321.
Lindahl, T., et al. 1976 J. Mol. Biol. 102, 511.
Henle, W., et al. 1967 Science 157, 1064.产品信息 形式 Lyophilized 配方 Lyophilized from 20 mM ammoinum bicarbonate, 100µg BSA. 负控制 HL60 cells 正控制 Raji cells 防腐剂 None 应用 应用参考 Original Clone Hearing, J.C., et al. 1985. Virol. 142, 215. 主要应用 Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
生物信息 免疫原 EBNA protein 克隆 E8.26 宿主 Mouse 同种型 IgG₁ 品种 EBV-infected mammalian B lymphocytes 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 +2°C to +8°C
无需冷冻 No
特殊指南 Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
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