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描述 产品目录编号 CC1026 品牌系列 Chemicon®
商名 - Chemicon
描述 Human Integrin α5β1, Octyl-β-D-glucopyranoside Formulation 概述 Integrin alpha5/beta1 purified from smooth muscle and placental tissue under nondenaturing conditions by affinity chromatography on fibronectin-Sepharose™ (Belkin, V. M. et al., 1990). Human tissues have been tested negative for HIV-1, HIV-2, HTLV-1, HTLV-2, Treponema pallidum, Hepatitis B and Hepatitis C. Alternate Names - VLA-5
背景信息 Integrin alpha5/beta1 purified from smooth muscle and placental tissue under nondenaturing conditions by affinity chromatography on fibronectin-Sepharose™ (Belkin, 1990). Human tissues have been tested negative for HIV-1, HIV-2, HTLV-1, HTLV-2, Treponema pallidum, Hepatitis B and Hepatitis C. This format of aVb5 is appropriate for functional evaluations. 产品信息 演示 Purified protein in 25 mM Tris, pH 7.4, 150 mM NaCl, 100 mM Octyl-b-D-glucopyranoside, 1mM MgCl2, 0.2 mM CaCl2 with 0.1% sodium azide. This format of alpha5beta1 is appropriate for functional evaluations. 应用 主要应用 - Positive Control
应用说明 Electrophoresis and Immunoblotting control. Due to the existance of reduction sensitive sites within the integrin subunits, it is recommended that gels be run under non-reducing conditions for best results. Under non-reducing conditions, the alpha5 subunit will migrate at 155 kDa while the beta1 subunit will migrate at 110 kDa.
Function: Liposomes containing Integrin alpha5/beta1 adhere to surfaces coated with fibronectin in an RGD-dependent fashion.
Optimal working dilutions must be determined by end user.生物信息 浓缩 0.36 mg/mL 纯度 Nonreduced SDS-PAGE of 15 μg on 5-15% polyacrylamide gel and staining with Coomassie shows only two main bands at 110 kDa (beta1 subunit) and 155 kDa (alpha5 subunit). A weak band at 205 kDa is due to dimerization of the beta1 subunit (Belkin, 1996).
CONTAMINANT(S):
None identified.来源 Human smooth muscle and placental tissue. 品种 Human Entrez基因编号 - NM_002205.2
Entrez基因汇总 The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. 基因符号 - FNRA
- ITGA5
- VLA-5
- CD49e
- VLA5A
UniProt编号 - P08648
UniProt汇总 FUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1049 amino acids; 114536 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -70°C in undiluted aliquots for up to 6 months after date of receipt. Thaw at 4°C. Avoid repeated freeze/thaw cycles.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.包装信息 数量 25 µg
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