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主要应用 CULT 描述 产品目录编号 CC078 品牌系列 Chemicon®
商名 - Chemicon
描述 Collagen Type III, bovine purified protein 概述 Molecular composition: a native triple helix, alpha1(III). Dissolved collagen retains the ability to form microfibrils, activate platelet aggregation and serve as a substrate for cell growth in culture. 产品信息 演示 Lyophilized protein containing no preservatives.
Recommended reconstitution instructions.
Add lyophilized collagen to 0.5 M acetic acid (3% glacial acetic acid v/v in water), 0.8 mL of solution to each mg of collagen.
Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension.)
Add acetic acid solution to dissolved collagen until solution is 1 mg/ml collagen. For large volumes, use a volumetric flask.应用 主要应用 - Cell Culture
生物信息 浓缩 Starting concentration is recommended at 1 mg/mL 纯度 90% by reduced SDS-PAGE. Contains 10% bovine collagen type I and < 0.5% non-collagen proteins. Structure of native collagen confirmed by ability to form microfibrils. 来源 Bovine skin. Collagen is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. 品种 Bovine 纯化方法 Differential salt precipitation. UniProt编号 - P04258
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20° C up to one year from date of receipt. Upon reconstitution, maintain at -20° C in undiluted aliquots. Avoid repeated freeze/thaw cycles. 包装信息 数量 10 mg
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