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merck millipore,默克密理博,CBL208,Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8
产品名称：Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8
产品型号：CBL208
Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
merck millipore,默克密理博,CBL208,Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 CBL208 Replaces MABT150 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 背景信息 IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. 产品信息 格式 Purified 控制 - Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
演示 Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water. 应用 应用 Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Electrophoretically purified cytokeratin 20 from human intestinal mucosa 克隆 IT-Ks20.8 宿主 Mouse 特异性 The antibody is specific for the 46 kDa polypeptide (cytokeratin 20). 同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_019010.1
Entrez基因汇总 The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. 基因符号 - MGC35423
- KRT20
- K20
- CK20
- Keratin-20
- CK-20
- KRT21
- Cytokeratin-20
- CD20
非反应性品种 - Rat
- Mouse
UniProt编号 - P35900
UniProt汇总 FUNCTION: SwissProt: P35900 # Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
SIZE: 424 amino acids; 48487 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Associates with KRT8.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa,and in the taste buds of lingual mucosa.
DEVELOPMENTAL STAGE: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low- differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
PTM: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). & Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
SIMILARITY: SwissProt: P35900 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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