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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS80 描述 Anti-phospho-TNK1 (Tyr277) Antibody Alternate Names - CD38 negative kinase 1
- Non-receptor tyrosine-protein kinase TNK1
背景信息 TNK1 (thirty-eight-negative kinase 1) is a non-receptor tyrosine kinase that is widely used in fetal development signaling and more narrowly used in adult tissues and lymphohematopoietic system cells. TNK1 seems to have regulatory ability on whether TNFalpha activates NF-kappaB or apoptosis. TNK1 can also inhibit Ras function and tumor growth. Mice with a TNK1 deficiency often show unprompted tumor growth, including lymphomas and carcinomas. TNK1 downregulation has also been shown in a large number of humans with diffuse large B-cell lymphoma. 产品信息 格式 Affinity Purified 控制 - PMA treated and untreated Molt-4 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho-TNK1 (Tyr277) Antibody is validated for use in WB for the detection of phospho-TNK1 (Tyr277). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human TNK1 phosphorylated at Tyr277. 表位 Phosphorylated Tyr277 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes TNK1 phosphorylated at Tyr277. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human and Mouse. Predicted to react with Rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003976
Entrez基因汇总 TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC (MIM 190090) and JAK (see MIM 147795) families, mediate intracellular signaling downstream of receptor activation.[supplied by OMIM]. 基因符号 - TNK1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - Q13470
UniProt汇总 FUNCTION: Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with the SH3 domain of PLCG1 via its Pro-rich domain.
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein
TISSUE SPECIFICITY: Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain. Ref.4 PTM: Autophosphorylated on tyrosine residues.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.产品使用声明 质量保证 Evaluated by Western Blot in PMA treated and untreated Molt-4 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected TNK1 on 10 µg of PMA treated and untreated Molt-4 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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