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![merck millipore,默克密理博,CBL180,Anti-CD62E [ELAM-1] Antibody, clone 1.2B6](/e/upload/m_pic/cbl180_s001w%5Bcbl180_s001w-ALL%5D.jpg "merck millipore,默克密理博,CBL180,Anti-CD62E [ELAM-1] Antibody, clone 1.2B6")
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merck millipore,默克密理博,CBL180,Anti-CD62E [ELAM-1] Antibody, clone 1.2B6
产品名称：Anti-CD62E [ELAM-1] Antibody, clone 1.2B6
产品型号：CBL180
Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in ELISA, FC, IP, WB, IH.
merck millipore,默克密理博,CBL180,Anti-CD62E [ELAM-1] Antibody, clone 1.2B6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H IHC, ELISA, FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 CBL180 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 Alternate Names - E-selectin
产品信息 格式 Purified 控制 - POSITIVE CONTROL: human tonsil.
演示 Purified by Protein-A affinity chromatography. The monoclonal is presented as a liquid in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. 应用 应用 Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in ELISA, FC, IP, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Flow Cytometry
- Western Blotting
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistochemistry: acetone fixed sections or fresh frozen, not recommended for paraffin embedded tissues.
Inhibits adhesion of neutrophils, eosinophils, and skin-homing T-cells to activated endothelial cells [F(ab')2].
Flow Cytometry: 0.5-2 μg/ 106 activated endothelial cells.
Western Blot: Non-reduced only
ELISA on endothelial cells: 10 μg/mL
Immunoprecipitation
Optimal working dilutions and protocols must be determined by the end user.生物信息 免疫原品种 TNF-activated human umbilical vein endothelial cells. 克隆 1.2B6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. The antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab')2 fragments. Although previously thought to recognize only human CD62E, recent data (Goda et al., 1999) shows that this antibody also recognizes human CD62P binding to a common epitope on these members of the selectin family. 同种型 IgG1 品种反应性 PigHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000450.1
Entrez基因汇总 The endothelial leukocyte adhesion molecule-1 is expressed by cytokine-stimulated endothelial cells. It is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. This gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. 基因符号 - ELAM1
- SELE
- LECAM2
- ESEL
- ELAM
- ELAM-1
- CD62E
UniProt编号 - P16581
UniProt汇总 FUNCTION: SwissProt: P16581 # Expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by ELAM-1 is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids).
SIZE: 610 amino acids; 66655 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P16581 ## Belongs to the selectin/LECAM family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 6 Sushi (CCP/SCR) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at +4°C for up to 6 months from the date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C. AVOID REPEATED FREEZE-THAW CYCLES. 包装信息 数量 100 µg
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