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品种反应性 主要应用 宿主 格式 抗体类型 M, H IH(P) Rb Purified Polyclonal Antibody 描述 产品目录编号 AB855 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-15 Antibody Alternate Names - MT2-MMP
产品信息 格式 Purified 控制 - POSITIVE CONTROL: Placenta
演示 Purified from rabbit anti-serum by Protein A chromatography. Prepared at 1mg/ml in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide 应用 应用 This Anti-MMP-15 Antibody is validated for use in IH(P) for the detection of MMP-15. 主要应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistology (formalin/paraffin) (Use AB at 2-4μg/ml for 30 min at RT)
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.生物信息 免疫原品种 A synthetic peptide from the mouse MMP-15/MT2-MMP protein. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB855 recognizes 62-68 kDa forms of a protein identified as MT2-MMP or MMP15. MMps are a group of enzymes involved in matrix degradation. To date there are sixteen members reported to belong to the family of matrix metalloproteinases. Of these sixteen proteins ten exist in soluble form whereas MT-MMPs exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT2-MMP plays a minor role in activation of pro-MMP-2 to its active form in breast carcinomas. Coexpression of MT1-MMP and MT2MMp in advanced stages of breast carcinomas is indicative of possible involvement of MT2-MMP in its metastsis.
CELLULAR LOCALIZATION: Cell Membrane品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002428.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. 基因符号 - EC 3.4.24.-
- MMP15
- MTMMP2
- MT2-MMP
- MT2MMP
- MMP-15
- SMCP-2
UniProt编号 - P51511
UniProt汇总 FUNCTION: SwissProt: P51511 # Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 669 amino acids; 75807 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential).
TISSUE SPECIFICITY: Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
DOMAIN: SwissProt: P51511 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store vial at 4ºC. When stored at 2-8ºC, this antibody is stable for 12 months. 包装信息 数量 100 µg
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