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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 CBL154 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-H-CAM Antibody, clone F10-44-2 Alternate Names - CD44
产品信息 格式 Purified 控制 - POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex
演示 The monoclonal is presented at a concentration of 100μg/mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. 应用 应用 This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).
Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.
Studies on HCAM (homing receptor) function.
Immunoprecipitation.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Purified human T cells 克隆 F10-44-2 宿主 Mouse 特异性 The CD44 antigen is a transmembranous glycoprotein of 80 kDa, which has undergone both extensive O-linked and N-linked glycosylation. This antibody is specific for epitope 1. The CD44 antigen is present on T lymphocytes, granulocytes, red blood cells, brain and epithelial cells. There is weak expression on platelets.
FUSION PARTNER: NS1 myeloma cell line同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001001392.1
- NM_000610.3
- NM_001001389.1
- NM_001001390.1
- NM_001001391.1
Entrez基因汇总 The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. 基因符号 - HCELL
- CD44
- LHR
- HUTCH-I
- CDW44
- CDw44
- ECMR-III
- Epican
- MGC10468
- CD44R
- MDU2
- PGP-1
- CSPG8
- Pgp1
- IN
- MUTCH-I
- MDU3
- MIC4
- MC56
UniProt编号 - P16070
UniProt汇总 FUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
SIZE: 742 amino acids; 81554 Da
SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. 包装信息 数量 100 µg
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