merck millipore,默克密理博,CBA071,CREB ELISA Kit

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,CBA071,CREB ELISA Kit
产品名称：CREB ELISA Kit
产品型号：CBA071

merck millipore,默克密理博,CBA071,CREB ELISA Kit

产品介绍

merck millipore,默克密理博,CBA071,CREB ELISA Kit

重要规格表

检测方法
Colorimetric

描述
概述	Detects and quantifies the level of CREB (cAMP-Response Element-Binding protein) independent of its phosphorylation state in human and mouse cells. CREB is a member of the leucine zipper family of DNA binding proteins. It induces the transcription of target genes in response to several stimuli such as peptide hormones and growth factors.
产品目录编号	CBA071
品牌系列	Calbiochem®
应用数据	The sensitivity of this ELISA was compared to immunoblotting using known quantities of CREB. The data presented in Figure 1 show that the sensitivity of the ELISA is ~4x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-CREB antibody and, chemiluminescent detection.

参考
参考	Impey, S. and Goodman, R. H. 2001. Sci. STKE. 82, PE1. Seternes, O.M., et al. 1999 Mol. Endocrinol. 13, 1071. Haywitz, A.J., and Greenberg, M.E. 1999. Annu. Rev. Biochem. 68, 821. Xing, J., et al. 1998. Mol. Cell. Biol. 18, 1946. Maldonado, R., et al. 1996. Science 273, 611. Gonzales, G.A. and Montminy, M.R. 1989. Cell 59, 675.

产品信息
检测方法	Colorimetric
形式	96 Tests
格式	96-well plate
试剂盒包含	CREB Standard, Standard Diluent Buffer, CREB Antibody-Coated 96-Well Plate, Rabbit Anti-CREB (Total) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.
正控制	HeLa, Jurkat, HT1080, and NIH3T3 cells

生物信息
化验范围	0.156-10 ng/ml
化验时间	4 h
样品类型	Cells
品种	human, mouse

理化信息
灵敏度	< 0.025 ng/ml

安全信息
R相	R: 25-36/37/38 Toxic if swallowed. Irritating to eyes, respiratory system and skin.
S相	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

产品使用声明
预定用途	The Calbiochem^® CREB ELISA Kit is designed to detect and quantify the level of CREB protein independent of its phosphorylation state. This assay also displays moderate cross reactivity to a related family member, CREM. This assay is intended for the detection of CREB from lysates of human and mouse cells. This kit can be used to normalize the phosphorylated CREB content of the samples when using the Calbiochem^® PhosphoDetect™ CREB (pSer¹³³) ELISA Kit (Cat. No. CBA072).

产品使用声明

预定用途

The Calbiochem^® CREB ELISA Kit is designed to detect and quantify the level of CREB protein independent of its phosphorylation state. This assay also displays moderate cross reactivity to a related family member, CREM. This assay is intended for the detection of CREB from lysates of human and mouse cells. This kit can be used to normalize the phosphorylated CREB content of the samples when using the Calbiochem^® PhosphoDetect™ CREB (pSer¹³³) ELISA Kit (Cat. No. CBA072).

储存和货运信息
货运代码	Blue Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	+2°C to +8°C
存储条件	Upon arrival store the entire contents of the kit at 4°C.
无需冷冻	No

补充信息
试剂盒包含	CREB Standard, Standard Diluent Buffer, CREB Antibody-Coated 96-Well Plate, Rabbit Anti-CREB (Total) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.