merck millipore,默克密理愽
  • merck millipore,默克密理博,17-360,Raf-1 Kinase Assay Kit, Chemiluminescence Detection

  • merck millipore,默克密理博,17-360,Raf-1 Kinase Assay Kit, Chemiluminescence Detection

    产品名称：Raf-1 Kinase Assay Kit, Chemiluminescence Detection
    产品型号：17-360
    Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user.

    merck millipore,默克密理博,17-360,Raf-1 Kinase Assay Kit, Chemiluminescence Detection

  • 产品介绍
  • merck millipore,默克密理博,17-360,Raf-1 Kinase Assay Kit, Chemiluminescence Detection

    重要规格表

    主要应用 可用的分析物 检测方法
    KARaf-1Chemiluminescence
    描述
    产品目录编号 17-360
    品牌系列 Upstate
    商名
    • Upstate
    描述 Raf-1 Kinase Assay Kit, Chemiluminescence Detection
    概述 Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user. For assay of Raf-1 activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-Raf-1 antibody (07-396).
    产品信息
    部件
    • Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Cat.# 07-461)
    • Raf-1 (truncated), active (Cat.# 14-352)
    • MEK1, unactive (Cat.# 14-420)
    • Magnesium/ATP Cocktail (Cat.# 20-113)
    • Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
    检测方法 Chemiluminescence
    应用
    应用 Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user.
    主要应用
    • Kinase Assay
    生物信息
    可用的分析物
    • Raf-1
    Entrez基因编号
    • NM_002880.2
    Entrez基因汇总 Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
    基因符号
    • C-RAF
    • RAF1
    • cRaf
    • Raf-1
    • c-Raf
    • RAF
    • CRAF
    UniProt编号
    • P04049
    UniProt汇总 FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
    COFACTOR: Binds 2 zinc ions per subunit.
    SIZE: 648 amino acids; 73052 Da
    SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
    PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
    产品使用声明
    质量保证 phosphorylation of MEK1
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit
    包装 Kit capacity: 50 kinase assays per kit

    merck millipore,默克密理博,17-360,Raf-1 Kinase Assay Kit, Chemiluminescence Detection

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