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merck millipore,默克密理博,AP342P-50ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP
产品名称：Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP
产品型号：AP342P-50ML
Detect Rabbit IgG using this Goat anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP validated for use in IH, IH(P).
merck millipore,默克密理博,AP342P-50ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP
- 产品介绍
描述 产品目录编号 AP342P-50ML 品牌系列 Chemicon®
商名 - Chemicon
描述 Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP 概述 Affinity purified goat anti rabbit IgG, polymeric HRP linked antibody. 背景信息 CHEMICON's IHC Amplification reagent utilizes a novel controlled polymerization technology to prepare polymeric AP-linker antibody conjugates. The reagent is derived from small, multifunctional, polymerizable linkers that are used to activate a mixture of enzymes and linker antibodies, with polymerization under controlled conditions. Although the end product is a heterogeneous conjugate, with different molecular sizes, shapes and enzyme/antibody ratios, all results suggest that knowledge of the those parameters seems not to be critical with regard to performance. Compared to conventional biotin-streptavidin based three-step kits, the IHC Amplification reagent has the advantage of simpler and faster protocols, higher amplification power, biotin free and more consistent immunostaining outcomes on a variety of tissue types and antibodies (Shi et al. 1999). 产品信息 格式 HRP 演示 Liquid. 应用 应用 Detect Rabbit IgG using this Goat anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP validated for use in IH, IH(P). 主要应用 - Immunohistochemistry
应用说明 PROTOCOL:
Immunohistochemistry: (All procedures should be performed at room temperature)
· Deparaffinization, rehydration
· Block endogenous peroxidase with 3% H2O2 in dd water for 10 minutes.
· Perform antigen retrieval or enzyme digestion if necessary.
1. Block with blocking solution, 10 minutes. Blot off blocking solution. No need to wash.
2. Incubate the rabbit primary antibody for 30-60 minutes, wash with PBS or TBS for 5 minutes, two times. (Incubation times with the primary antibody will need to be determined by the end user.)
3. Incubate with the ready-to-use Poly-HRP-Anti-Rabbit IgG for 30 minutes, wash with PBS or TBS for 5 minutes, two times.
4. Incubate with DAB or AEC solution for 5 minutes, wash with dd water.
5. Counterstain with hematoxylin and mount.
NOTES:
1. The system is biotin free so that blocking endogenous biotin is not necessary.
2. A pre-primary antibody blocking with 10% normal goat serum is recommended (CHEMICON catalog number S26-100ML).生物信息 浓缩 ready to use 宿主 Goat 品种反应性 Rabbit 抗体类型 Polyclonal Antibody 纯化方法 Immunoaffinity purified 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain product at 2-8°C for up to 12 months.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.包装信息 数量 50 mL
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