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merck millipore,默克密理博,AP1063,PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb
产品名称：PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb
产品型号：AP1063
This PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).
merck millipore,默克密理博,AP1063,PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells. 产品目录编号 AP1063 品牌系列 Calbiochem®
同义词 Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb 应用数据 
Detection of human PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunoblotting. Samples: Whole tissue extract from mouse liver (20 µg) left untreated (lane 1) or treated with dichloroacetate (DCA, 5 mM for 4 h) (lane 2). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) PhosphoDetect™ Anti PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) PhosphoDetect™ Anti PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) all used at 0.25 µg/ml and Anti-PDHE-1α. Detection: chemiluminescence. Data courtesy of Matthew Rardin, University of California San Diego.
Detection of PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunocytochemistry. Samples: COS7 cells incubated in presence dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker® Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween® 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (panel B), PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (panel C), PhosphoDetect™ Anti-PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) (panel D) (0.5 µg/ml, green fluorescence) and anti-PDH-E1α antibody (green fluorescence, top 3 panels) (panel A). Detection: fluorescence (Alexa Fluor® 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Matthew Rardin, University of California San Diego.参考 参考 Rardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.产品信息 形式 Liquid 配方 In PBS. 正控制 HEK293 cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α 免疫原品种 Human 宿主 Rabbit 品种 human, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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