- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Chp, H, M, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS95 描述 Anti-NDK A Antibody Alternate Names - nm23-H1
- Nucleoside diphosphate kinase A
- NDK A
- NDP kinase A
- Granzyme A-activated Dnase
- GAAD
- Metastasis inhibition factor nm23
- Tumor metastatic process-associated protein
背景信息 NME1/NDK A is a metastasis-suppressor protein that has been found to be down-regulated in human lung cancer cells. Polymorphisms in NDK A have shown a significant association with propensity and severity of lung cancer, breast cancer and other forms of human cancer. NDK A is known as a tumor metastasis suppressor that works on a wide range of tumors. Recently, NDK A has also been found to regulate subcellular trafficking of annexin IV and EF-1Bα. 产品信息 格式 Affinity Purified 控制 - MCF7 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-NDK A Antibody is an antibody against NDK A for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human NDK A. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 ChimpanzeeHumanMouseRat Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000260
Entrez基因汇总 This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]. 基因符号 - NM23
- NME1
- NDPKA
纯化方法 Affinity purified UniProt编号 - P15531
UniProt汇总 FUNCTION: Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
CATALYTIC ACTIVITY: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
COFACTOR: Magnesium.
ENZYME REGULATION: Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity.
SUBUNIT STRUCTURE: Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
TISSUE SPECIFICTY: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed. MISCELLANEOUS: The role of this protein in tumor development and progression is uncertain. This protein is found in reduced amount in some tumor cells of high metastatic potential. However, increased NME1 levels correlate with aggressive tumor features in neuroblastoma. May have distinct if not opposite roles in different tumors.
SEQUENCE SIMILARITIES: Belongs to the NDK family.
SEQUENCE CAUTION: The sequence CAA35621.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected NDK A on 10 µg of MCF7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MABF336,Anti-CD123 (human) Antibody, FITC, clone 6H6
下一件merck millipore产品：merck millipore,默克密理博,71900,NanoJuice Core Transfection Reagent
相关产品
merck millipore,默克密理博,SF-113,TWIST; Mouse, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,IHCR1003-6,Anti-Tyrosine Hydroxylase Antibody, prediluted
merck millipore,默克密理博,14-582,FGFR1 Protein, active, 10 µg
merck millipore,默克密理博,FCABS325PE,Milli-Mark™ Anti-acetyl-Histone H3-PE Antibody
merck millipore,默克密理博,MABF336,Anti-CD123 (human) Antibody, FITC, clone 6H6
merck millipore,默克密理博,71900,NanoJuice Core Transfection Reagent
merck millipore,默克密理博,12-426,Phospho (Ser10)-acetyl (Lys14)-Histone H3 Peptide, biotin conjugate
merck millipore,默克密理博,ABC273,Anti-SLC22A18 Antibody
merck millipore,默克密理博,FTLV00003,Fast-Trap Lentivirus Purification and Concentration Kit
merck millipore,默克密理博,GF313,TGF- α Protein, Human Recombinant Animal Free