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品种反应性 主要应用 宿主 格式 抗体类型 Eq, H, M, R, B WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS93 描述 Anti-RAB25 Antibody Alternate Names - CATX-8
- Ras-related protein Rab-25
背景信息 Ras-related protein Rab-25 (RAB25) is a GTP-binding protein belonging to the Rab family of proteins. RAB25 has been observed to be expressed in kidney, lung and gastrointestinal mucosa, with exclusive expression in epithelial cells. This specific expression has led to the implication that RAB25 has a role as a regulator for vesicular transport associated with epithelia. RAB25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have observed a loss of RAB25 in breast cancer tissue and cell lines, and have suggested a potential role for RAB25 as a regulator for Ras induced tumorigenesis. 产品信息 格式 Affinity Purified 控制 - MCF7 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide. 应用 应用 Anti-RAB25 Antibody detects level of RAB25 & has been published & validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:600 dilution of a representative lot of this antibody detected RAB25 in invasive and in situ ductal carcinoma tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human RAB25. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 HorseHumanMouseRatBovine Species Reactivity Note Demonstrated to react with Human, Mouse and Rat.
Predicted to react with Bovine and Equine based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_065120
Entrez基因汇总 Summary: RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM]. 基因符号 - CATX8
- RAB25
纯化方法 Affinity Purified UniProt编号 - P57735
UniProt汇总 FUNCTION: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling and/or transcytotic pathways.
SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (potential). Cell projection › pseudopodium membrane. Cytoplasmic vesicle. Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
TISSUE SPECIFICITY: Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.
SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Rab family.
SEQUENCE CAUTION: The sequence AAF98238.1 differs from that shown. Reason: Frameshift at position 196. The sequence AAM69362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected RAB25 in 10 µg of MCF7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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