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品种反应性 主要应用 宿主 格式 抗体类型 R, M ICC, WB Gp Serum Polyclonal Antibody 描述 产品目录编号 ABN90 描述 Anti-NeuN Antibody 背景信息 ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. 产品信息 格式 Serum 控制 - Mouse brain E16 tissue lysate
演示 Guinea pig polyclonal serum containing 0.05% sodium azide. 应用 应用 Detect NeuN using this Anti-NeuN Antibody validated for use in Western Blotting, IHC(P), ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:3,000 dilution from a representative lot detected NeuN in normal mouse cerebellum and frontal cortex tissues.
Immunocytochemistry Analysis: A 1:8,000 dilution from a representative lot detected NeuN in Rat E18 cortex and hippocampus primary cultures.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN. 表位 N-terminus 宿主 Guinea Pig 特异性 This antibody recognizes the N-terminus of NeuN. 品种反应性 RatMouse Species Reactivity Note Demonstrated to react with Mouse and Rat. 抗体类型 Polyclonal Antibody 纯化方法 Unpurified 产品使用声明 质量保证 Evaluated by Western Blot in mouse brain E16 tissue lysate.
Western Blot Analysis: A 1:4,000 dilution of this antibody detected NeuN in 10 µg of mouse brain E16 tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,05-368MG,Anti-phospho-Ser/Thr-Pro MPM-2 Antibody
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