merck millipore,默克密理博,ABE223-S,Anti-acetyl Histone H3 (Lys4) Antibody, Trial Size

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABE223-S
描述	Anti-acetyl Histone H3 (Lys4) Antibody, Trial Size
Alternate Names	h3K4Ac Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/I Histone H3/j Histone H3/k Histone H3/l
背景信息	Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, H3K4 is acetylated by Gcn5 and Rtt109. H3K4Ac is found at the promoters of active genes.

产品信息
格式	Affinity Purified
控制	Sodium butyrate treated HeLa acid extract
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Use Anti-acetyl Histone H3 (Lys4) Antibody (Rabbit Polyclonal Antibody), Trial Size / Pack validated in WB, IP, DB to detect acetyl Histone H3 (Lys4) also known as Histone H3.1.
主要应用	Western Blotting
应用说明	Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-acetyl-Histone H3 (Lys4). Western Blotting Analysis: Representative lot data. Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract. Cat. No. ABE223, Anti-acetyl Histone H3 (Lys4) (1:1000 dilution). detected Histone H3 acetylated on Lys4 in 10 µg of sodium butyrate treated HeLa acid extract. Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates acetyl Histone H3 (Lys4) (~17 kDa).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys4.
表位	Acetylated Lys4
宿主	Rabbit
特异性	This antibody recognizes Histone H3 acetylated at Lys4.
品种反应性	RatHumanMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_003520
基因符号	H3FJ HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J
纯化方法	Affinity purified
UniProt编号	P68431

产品使用声明
质量保证	Evaluated by Western Blot in sodium butyrate treated HeLa acid extract. Western Blot Analysis: A 1:1,000 dilution of this antibody detected Histone H3 acetylated on Lys4 in 10 µg of sodium butyrate treated HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	10 µL

merck millipore,默克密理博,ABE223-S,Anti-acetyl Histone H3 (Lys4) Antibody, Trial Size

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