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品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, H, R, Chp IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE198 描述 Anti-Sirt5 Antibody Alternate Names - SIR2-like protein 5
- NAD-dependent deacetylase sirtuin-5
背景信息 Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-Sirt5 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Sirt5 also known as NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected Sirt5 in convoluted tubules of normal rat kidney tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Sirt5. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Rhesus MacaqueHumanRatChimpanzee Species Reactivity Note Demonstrated to react with Human and Rat. Predicted to react with Chimpanzee and Rhesus Macaque based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036373
Entrez基因汇总 Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]. 基因符号 - SIR2L5
- SIRT5
纯化方法 Affinity purified UniProt编号 - Q9NXA8
UniProt汇总 FUNCTION: NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting. Deacetylates CPS1 and increases CPS1 activity in response to elevated NAD levels during fasting (by similarity). Can deacetylate cytochrome c and a number of other proteins (in vitro).
CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
COFACTOR: Binds 1 zinc ion per subunit.
ENZYME REGULATION: Inhibited by suramin.
SUBUNIT STRUCTURE: Monomer. Homodimer. Forms homodimers upon suramin binding. Interacts with CPS1 (by similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix. Mitochondrion intermembrane space (potential).
TISSUE SPECIFICITY: Widely expressed.
SEQUENCE SIMILARITIES: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Sirt5 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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