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merck millipore,默克密理博,ABE10,Anti-monomethyl Histone H3 (Arg8) Antibody
产品名称：Anti-monomethyl Histone H3 (Arg8) Antibody
产品型号：ABE10
Detect monomethyl Histone H3 (Arg8) with Anti-monomethyl Histone H3 (Arg8) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB, PIA.
merck millipore,默克密理博,ABE10,Anti-monomethyl Histone H3 (Arg8) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE10 描述 Anti-monomethyl Histone H3 (Arg8) Antibody Alternate Names - H3 histone family, member T
- histone cluster 3, H3
- histone 3, H3
- histone H3.1t
背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 methylated at Arg8 is involved in nuclear-receptor-mediated transcriptional activation. 产品信息 格式 Affinity Purified 控制 - HeLa acid extract.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect monomethyl Histone H3 (Arg8) with Anti-monomethyl Histone H3 (Arg8) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB, PIA. 主要应用 - Western Blotting
应用说明 Peptide Inhibition Assay: Specificity of a previous lot confirmed by the ability of 0.1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts (Lane 3). No signal reductions were detected with preincubation with 10 μM of unmodified, symmetrically dimethylated or asymmetrically dimethylated arginine 8 modified peptides. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 methylated at Arg8. 表位 Methylated Arg8 宿主 Rabbit 特异性 This antibody recognizes the N-terminus of Histone H3 when methylated at Arg8. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Predicted broad species reactivity based on sequence similarity. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003484.1
Entrez基因汇总 Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]. 基因符号 - H3T
- H3/t
- H3t
- H3/g
- H3FT
- H3.4
修改 - Methylation
纯化方法 Affinity purified UniProt编号 - Q16695
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus. Chromosome.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
POST-TRANSLATIONAL MODIFICATION: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me) (by similarity). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription (by similarity). Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (by similarity). Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin (by similarity). Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (by similarity). Ubiquitinated (by similarity).
SEQUENCE SIMILARITIES: Belongs to the histone H3 family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected monomethyl Histone H3 (Arg8) on 10 µg of HeLa acid extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µL
上一件merck millipore产品：merck millipore,默克密理博,CBL434,Anti-c-myc Antibody, clone 3C7
下一件merck millipore产品：merck millipore,默克密理博,07-274,Anti-dimethyl-Histone H3 (Lys36) Antibody
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