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品种反应性 主要应用 宿主 格式 抗体类型 M WB, ICC, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB9878 商名 - Chemicon
描述 Anti-MAGI-2 Antibody Alternate Names - Synaptic Scaffolding Molecule
- Membrane Associated Guanylate Kinase, WW and PDZ domain containing 2
- S-SCAM
- S-SCAM
背景信息 The spatial coordination of neurotransmitter receptors with other postsynaptic signaling and structural molecules is regulated by a diverse array of cell-specific scaffolding proteins. The synaptic trafficking of AMPA receptors by the stargazin protein in some neurons, for example, depends on specific interactions between the C terminus of stargazin and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of membrane-associated guanylate kinase scaffolding proteins PSD-93 or PSD-95. Stargazin [Cacng2 (Ca2+ channel gamma2 subunit)] is one of four closely related proteins recently categorized as transmembrane AMPA receptor regulating proteins (TARPs) that appear to share similar functions but exhibit distinct expression patterns in the CNS. MAGI-2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) has been identified as a novel candidate interactor with the cytoplasmic C termini of the TARPs. MAGI-2 [also known as S-SCAM (synaptic scaffolding molecule)] is a multi-PDZ domain scaffolding protein that interacts with several different ligands in brain, including PTEN (phosphatase and tensin homolog), dasm1 (dendrite arborization and synapse maturation 1), dendrin, axin, beta- and delta-catenin, neuroligin, hyperpolarization-activated cation channels, beta1-adrenergic receptors, and NMDA receptors. MAGI-2 coimmunoprecipitates with stargazin in vivo. Expression of stargazin recruites MAGI-2 to cell membranes and cell-cell contact sites in transfected HEK-293T cells dependent on the presence of the stargazin -TTPV motif. MAGI-2 represents a strong candidate for linking TARP/AMPA receptor complexes to a wide range of other postsynaptic molecules and pathways and advance our knowledge of protein interactions at mammalian CNS synapses. 产品信息 格式 Purified 控制 - Mouse brain
演示 Purified antibody in 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3. 应用 应用 This Anti-MAGI-2 Antibody is validated for use in IC, IH, IP, WB for the detection of MAGI-2. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Optimal working dilutions must be determined by the end user. 生物信息 免疫原品种 GST-tagged MAGI-2 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Cat. # AB9878 recognizes MAGI-2 品种反应性 Mouse Species Reactivity Note Mouse. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036433.2
Entrez基因汇总 The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq] 基因符号 - SSCAM
- MAGI2
- ACVRINP1
- AIP1
纯化方法 Protein A purfied UniProt编号 - O88382
UniProt汇总 Function: Seems to act as scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins. May play a role in regulating activin-mediated signaling in neuronal cells. Enhances the ability of PTEN to suppress AKT1 activation By similarity.
Subunit structure: Interacts through guanylate kinase domain its with DLGAP1. Interacts through the PDZ domains with GRIN2A, GRID2 and NLGN1. Interacts with CTNND2 and MAGUIN-1. Interacts with CTNNB1, ACVR2A, SMAD2 and SMAD3 By similarity. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3 By similarity. Interacts via its WW domains with DRPLA By similarity. Interacts via its second PDZ domain with PTEN unphosphorylated C-terminus; this interaction diminishes the degradation rate of PTEN. May interact with HTR2A and IGSF9 By similarity. Interacts with RAPGEF2. Interacts with HTR4 By similarity.
Subcellular location: Cell junction › synapse › synaptosome. Cell membrane; Peripheral membrane protein. Note= Membrane-associated in synaptosomes. Ref.1
Tissue specificity: Exclusively expressed in brain. Ref.1
Sequence similarities: Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains.产品使用声明 质量保证 Routinely evaluated by Western Blotting on mouse brain tissue lysate.
Western Blotting Analysis: 1:500 dilution of this lot was used to detect MAGI-2 on mouse brain lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.包装信息 数量 100 µg
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