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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Sh, B, Ch, H, M, R, Rb IHC, ELISA, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9746 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-14-3-3 ζ Antibody 背景信息 Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.
The 14-3-3 zeta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.产品信息 格式 Serum 演示 Liquid. 应用 应用 Detect 14-3-3 ζ using this Anti-14-3-3 ζ Antibody validated for use in ELISA, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Western blot: 1:3000 using ECL.
Immunohistochemistry: 1:200-1:400 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide from the N-terminal area of sheep 14-3-3 zeta. 宿主 Rabbit 特异性 14-3-3 zeta. By Western blot the antibody recognizes the ~30 kDa protein. 品种反应性 SheepBovineChickenHumanMouseRatRabbit 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_145690.1
- NM_003406.2
Entrez基因汇总 This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. 基因符号 - MGC138156
- YWHAZ
- KCIP-1
- MGC126532
- MGC111427
纯化方法 Serum UniProt编号 - P63104
UniProt汇总 FUNCTION: SwissProt: P63104 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
SIZE: 245 amino acids; 27745 Da
SUBUNIT: Homodimer. Interacts with PCTK1. Binds to the C-terminal part of WEE1. Interacts with BSPRY. Binds to phosphorylated MLF1 and retains it in the cytoplasm (By similarity). Interacts with MLLT7/FOXO4 and SSH1.
SUBCELLULAR LOCATION: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
PTM: Isoform delta differs from isoform zeta in being phosphorylated (By similarity).
SIMILARITY: SwissProt: P63104 ## Belongs to the 14-3-3 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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