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品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB9642 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dab1 phospho Serine 491 Antibody 背景信息 Dab1 is a 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically mutatation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by reelin. Dab1 serine 491 is phosphorylated in a Cdk5-dependant manner and regulates, likely indirectly, reelin-inducing signaling during neural cortex development. 产品信息 格式 Purified 控制 - POSITIVE CONTROL:
HEK293 cells transfected with wild-type mouse Dab1 protein.
演示 Protein A purified immunoglobulin. Liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide. 应用 应用 Detect Dab1 phospho Serine 491 using this Anti-Dab1 phospho Serine 491 Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:1,000. For use in detecting endogenous Dab1 protein it is recommended that the Dab1 protein first be immunoprecipitated.
Suggested blocking buffer is 5% BSA-TBST. Suggested blocking time is overnight at 2-8?C. Suggested antibody dilution buffer is 3% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide of amino acids surrounding the phosphoSerine 491 site of human Dab1. 宿主 Rabbit 特异性 Dab1 phosphoSerine 491. The antibody recognizes a protein of ~80 kDa corresponding to Dab1 pSer 491 in lysates from HEK293 transfected cells. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_021080.3
Entrez基因汇总 The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. 基因符号 - DAB1
修改 - Phosphorylation
UniProt编号 - O75553
UniProt汇总 FUNCTION: SwissProt: O75553 # Adapter molecule functioning in neural development. May regulate SIAH1 activity (By similarity).
SIZE: 588 amino acids; 63775 Da
SUBUNIT: Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1 (By similarity). Interacts with LRP1.
DOMAIN: SwissProt: O75553 The PID domain specifically binds to the Asn-Pro-Xaa- Tyr(P) motif found in many tyrosine-phosphorylated proteins.
PTM: Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser- 524 independently of reelin signaling (By similarity).
SIMILARITY: Contains 1 PID domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer. 包装信息 数量 100 µL
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