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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6039 描述 Anti-Vinculin Antibody Alternate Names - Metavinculin
背景信息 Cell spreading and movement occur though the process of binding of cell surface integrin receptors to extracellular matrix adhesion molecules. In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. More specifically, vinculin binds to talin at its N-terminus, which, in turn, binds to beta integrins. At its C-terminus, vinculin binds to actin, phospholipids, and paxillin-forming homodimers. The binding complex of vinculin to talin and actin serves to anchor actin filaments to the membrane, (Ezzell, 1997).
Vinculin's ability to interact with integrins at the focal adhesion appears to be critical for control of cytoskeletal mechanics, cell spreading, and lamellipodia formation. Thus, vinculin appears to play a key role in shape control based on its ability to modulate focal adhesion structure and function.产品信息 格式 Affinity Purified 控制 - HepG2 cell lysate.
演示 Purified in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Vinculin using this Anti-Vinculin Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to an internal domain of human Vinculin. 表位 Internal Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes an internal domain of human vinculin. 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, and rat. Predicted to cross react with monkey, pig, cow, and horse based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_054704
Entrez基因汇总 Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. 基因符号 - VCL
纯化方法 Antigen affinity purified UniProt编号 - P18206
UniProt汇总 FUNCTION: Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of adhesion plaques.
POST TRANSLATIONAL MODIFCIATION: Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.产品使用声明 质量保证 Evaluated on a representative lot by Western blot on HepG2 cell lysate using Anti-Vinculin. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µg
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