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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5756 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-LIM/Homeobox Protein Lhx2 Antibody Alternate Names - Homeobox Protein LH-2
产品信息 格式 Affinity Purified 演示 Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. 应用 应用 Anti-LIM/Homeobox Protein Lhx2 Antibody is an antibody against LIM/Homeobox Protein Lhx2 for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from human Lhx2. 宿主 Rabbit 特异性 Recognizes the LIM homeobox containing transcription factor Lhx2. 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004789.3
Entrez基因汇总 This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. 基因符号 - MGC138390
- LHX2
- LH-2
- LH2
- hLhx2
UniProt编号 - P50458
UniProt汇总 FUNCTION: SwissProt: P50458 # Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity).
SIZE: 406 amino acids; 44373 Da
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: SwissProt: P50458 ## Contains 1 homeobox DNA-binding domain. & Contains 2 LIM zinc-binding domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
PREPARATION AND USE:
To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.
For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.包装信息 数量 100 µg
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