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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5719-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-KCa3.1 Antibody Alternate Names - Intermediate conductance Ca2+-activated K+ Channel 4, SK4, IKCa1, KCNN4
- Intermediate conductance Ca2+-activated K+ Channel 4
- SK4
- IKCa1
产品信息 格式 Affinity Purified 控制 - Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 Anti-KCa3.1 Antibody detects level of KCa3.1 & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:300 using ECL on KCa3.1 transfected HEK-293 and K562 cells.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.
2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
5. Destain with deionized water.
6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.
8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.
9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.
10. Wash 4 x 10 min. with PBS-0.1% tween 20.
11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).生物信息 免疫原品种 Synthetic peptide from the C-terminal area of rat KCa3.1 (Accession Q9QYW1). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 KCa3.1 (Intermediate conductance Ca2+-activated K+ Channel4, IKCa1, SK4, KCNN4) 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002250.2
Entrez基因汇总 The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. 基因符号 - hKCa4
- KCNN4
- hIKCa1
- IK1
- IKCa1
- KCa4
- SK4
- KCA4
- KCa3.1
- hSK4
- IKCA1
UniProt编号 - O15554
UniProt汇总 FUNCTION: SwissProt: O15554 # Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
SIZE: 427 amino acids; 47696 Da
SUBUNIT: Heterotetramer of potassium channel proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in non-excitable tissues.
SIMILARITY: SwissProt: O15554 ## Belongs to the potassium channel KCNN family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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