merck millipore,默克密理愽
  • merck millipore,默克密理博,16-184,Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate

  • merck millipore,默克密理博,16-184,Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate

    产品名称：Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate
    产品型号：16-184
    Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in WB.

    merck millipore,默克密理博,16-184,Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate

  • 产品介绍
  • merck millipore,默克密理博,16-184,Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    AWBMHRPMonoclonal Antibody
    描述
    产品目录编号 16-184
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, HRP conjugate
    概述 Anti-phosphotyrosine antibody (05-321) cross-linked to horseradish peroxidase (HRP).
    背景信息 The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    Description
    Recombinant 4G10®, produced in Chinese Hamster Ovary (CHO) cells expressing the 4G10® antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine (His6) tag for purification and immobilization via nickel affinity matrices. Cross-linked to horseradish peroxidase (HRP). Patent pending.
    产品信息
    格式 HRP
    控制
    • A431 carcinoma cells.
      Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
    演示 IgG2bκ-HRP conjugate in PBS containing 0.05% ProClin®150, as a preservative.
    应用
    应用 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in WB.
    主要应用
    • Western Blotting
    应用说明 Recognizes tyrosine-phosphorylated proteins, useful in immunoblotting.
    生物信息
    免疫原品种 Phosphotyramine-KLH.
    表位 N/A
    克隆 4G10®
    宿主 Mouse
    特异性 Recognizes tyrosine-phosphorylated proteins from all species.
    同种型 IgG2bκ
    品种反应性 All
    Species Reactivity Note All
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • Varies
    修改
    • Phosphorylation
    纯化方法 Protein G purfied
    UniProt编号
    • Varies
    产品使用声明
    质量保证 Routinely evaluated in western blot to detect tyrosine-phosphorylated proteins in RIPA lysates from EGF-stimulated human A431 carcinoma cells.

    Western Blot Analysis:
    Tested at 1:2500-1:5000, this lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-stimulated human A431 carcinoma cells
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8°C from date of receipt.
    For maximum recovery of product, centrifuge the original vial prior to removing the cap.
    Note: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
    包装信息
    数量 10 blots

    merck millipore,默克密理博,16-184,Anti-Phosphotyrosine Antibody, recombinant clone 4G10, HRP conjugate

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