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  • merck millipore,默克密理博,AB5600-50UL,Anti-Synaptotagmin I Antibody, luminal part

  • merck millipore,默克密理博,AB5600-50UL,Anti-Synaptotagmin I Antibody, luminal part

    产品名称：Anti-Synaptotagmin I Antibody, luminal part
    产品型号：AB5600-50UL
    Detect Synaptotagmin I using this Anti-Synaptotagmin I Antibody, luminal part validated for use in WB, IH.

    merck millipore,默克密理博,AB5600-50UL,Anti-Synaptotagmin I Antibody, luminal part

  • 产品介绍
  • merck millipore,默克密理博,AB5600-50UL,Anti-Synaptotagmin I Antibody, luminal part

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    RIHC, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5600-50UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Synaptotagmin I Antibody, luminal part
    Alternate Names
    • Syt1
    • p65
    产品信息
    格式 Affinity Purified
    控制
    • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Detect Synaptotagmin I using this Anti-Synaptotagmin I Antibody, luminal part validated for use in WB, IH.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    应用说明 Western blot: 1:200-1:1,000 using ECL on rat brain membranes.

    Immunohistochemistry on rat brain sections.

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Peptide corresponding to amino acids 1-19 from rat Synaptotagmin I (Accession # P21107).
    表位 luminal part
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Synaptotagmin I, luminal part. The epitope does not share homology with any other known proteins.

    SPECIES REACTIVITIES: It is expected that the antibody will also recognize human. Other species have not been tested.
    品种反应性 Rat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_005639.1
    Entrez基因汇总 The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
    基因符号
    • Synaptotagmin-1
    • SYT1
    • DKFZp781D2042
    • SVP65
    • SytI
    • SYT
    • p65
    • P65
    UniProt编号
    • P21579
    UniProt汇总 FUNCTION: SwissProt: P21579 # May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
    COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains (By similarity).
    SIZE: 422 amino acids; 47573 Da
    SUBUNIT: Homotetramer (Probable). Interacts with STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity).
    SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note=Synaptic vesicles and chromaffin granules.
    DOMAIN: SwissProt: P21579 The first C2 domain mediates Ca(2+)-dependent phospholipid binding. & The second C2 domain mediates interaction with SV2A and STN2 (By similarity).
    SIMILARITY: Belongs to the synaptotagmin family. & Contains 2 C2 domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5600-50UL,Anti-Synaptotagmin I Antibody, luminal part

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