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描述 概述 The pET-44 vectors are designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag™) protein. The solubility of many target proteins in the E. coli cytoplasm is increased when they are expressed NusA fusions. The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag® sequence for enhanced yields when purifying a target protein with His•Mag™ Agarose Beads from lysates generated with the BugBuster® HT Reagent and PopCulture® Reagent. N-terminal S•Tag™ and optional C-terminal HSV•Tag® and His•Tag fusion sequences are also available for versatile detection and purification of target proteins.
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.产品目录编号 71122 品牌系列 Novagen®
储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
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