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品种反应性 主要应用 宿主 格式 抗体类型 R WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5592-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-GABA A Receptor α1 Antibody Alternate Names - gamma-aminobutyric acid receptor type A alpha1 subunit
产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 Detect GABA A Receptor α1 using this Anti-GABA A Receptor α1 Antibody validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot: 1:400-1:1000 using ECL on rat brain membranes. Recognizes a 51kDa band.
Immunohistochemistry on rat brain sections. 4% PFA fixation, frozen sections 1:100-1:200
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Peptide corresponding to amino acids 1-16 from mouse or rat GABA(A) alpha1 subunit (Swiss-Prot accession number P18504, residues 28-43 of the precursor). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes GABA(A) alpha1. The epitope shares little sequence similarity with alpha5 and alpha2 subunits (respectively, 9/16 and 8/16 residues identical). Does not share homology with any other known proteins.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse. The epitope is highly conserved in human and bovine (16/17) and chicken (14/17). Other species have not been tested.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000806.4
Entrez基因汇总 GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. 基因符号 - EJM
- GABRA1
- ECA4
- GABA
UniProt编号 - P14867
UniProt汇总 FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 456 amino acids; 51802 Da
SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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