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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5424P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Per1 Antibody Alternate Names - Period Circadian Protein 1
- RIGUI
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. 应用 应用 This Anti-Per1 Antibody is validated for use in ELISA, WB for the detection of Per1. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 μg/mL using ECL.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide (Catalog Number AG330) per well.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A 20 amino acid peptide sequence within the C-terminus of human Per1 (Sun et al. 1997; Tei et al. 1997). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes human Per 1 (RIGUI). The immunogen shows no significant sequence homology with Per 2, Per 3, dPer or other known proteins. The immunogen sequence has 100% homology with mouse and rat Per1 and 65% with chicken. Reactivity with other species has not been confirmed. Per 1 is a protein of ~136 kDa. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002616.1
Entrez基因汇总 This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described. 基因符号 - hPER
- PER1
- PER
- KIAA0482
- MGC88021
- RIGUI
- hPER1
UniProt编号 - O15534
UniProt汇总 FUNCTION: SwissProt: O15534 # Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK NPAS2-BMAL1 BMAL2-induced transactivation (By similarity).
SIZE: 1290 amino acids; 136238 Da
SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity).
TISSUE SPECIFICITY: Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney.
PTM: Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: O15534 ## Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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