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  • merck millipore,默克密理博,AB5164-200UL,Anti-Muscarinic Acetylcholine Receptor m1 Antibody

  • merck millipore,默克密理博,AB5164-200UL,Anti-Muscarinic Acetylcholine Receptor m1 Antibody

    产品名称：Anti-Muscarinic Acetylcholine Receptor m1 Antibody
    产品型号：AB5164-200UL
    This Anti-Muscarinic Acetylcholine Receptor m1 Antibody is validated for use in IH, WB for the detection of Muscarinic Acetylcholine Receptor m1. Less<<

    merck millipore,默克密理博,AB5164-200UL,Anti-Muscarinic Acetylcholine Receptor m1 Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5164-200UL,Anti-Muscarinic Acetylcholine Receptor m1 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5164-200UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Muscarinic Acetylcholine Receptor m1 Antibody
    产品信息
    格式 Affinity Purified
    控制
    • Included free of charge with the antibody is 60 μg of control fusion protein (lyophilized powder). The stock solution of the fusion protein can be made up using 100 μL of PBS. For positive control, in Western blot using 10 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
    应用
    应用 This Anti-Muscarinic Acetylcholine Receptor m1 Antibody is validated for use in IH, WB for the detection of Muscarinic Acetylcholine Receptor m1.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Western blot: 1:200 using ECL on rat brain membranes with proteinase inhibitors. M1 is approximately 60-80kDa in size, some preparations yield 125kDa dimers (photo). Heating samples to 80-90C rather than fulling boiling and adjusting sample pH to neutral can help in preventing dimer formation.

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Immunohistochemistry: rat frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only.



    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 GST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes a full length m1 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
    品种反应性 RatMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_000738.2
    Entrez基因汇总 The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
    基因符号
    • MGC30125
    • CHRM1
    • M1
    • HM1
    UniProt编号
    • P11229
    UniProt汇总 FUNCTION: SwissProt: P11229 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
    SIZE: 460 amino acids; 51421 Da
    SUBUNIT: Interacts with GPRASP2.
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    SIMILARITY: SwissProt: P11229 ## Belongs to the G-protein coupled receptor 1 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,AB5164-200UL,Anti-Muscarinic Acetylcholine Receptor m1 Antibody

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