merck millipore,默克密理博,AB5152-200UL,Anti-Calcium Channel Antibody, Voltage Gated α 1A

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merck millipore,默克密理博,AB5152-200UL,Anti-Calcium Channel Antibody, Voltage Gated α 1A
产品名称：Anti-Calcium Channel Antibody, Voltage Gated α 1A
产品型号：AB5152-200UL
This Anti-Calcium Channel Antibody, Voltage Gated α 1A is validated for use in IC, IP, WB for the detection of Calcium Channel.

merck millipore,默克密理博,AB5152-200UL,Anti-Calcium Channel Antibody, Voltage Gated α 1A

产品介绍

merck millipore,默克密理博,AB5152-200UL,Anti-Calcium Channel Antibody, Voltage Gated α 1A

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, M	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5152-200UL
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Calcium Channel Antibody, Voltage Gated α 1A
Alternate Names	P/Q-type

产品信息
格式	Affinity Purified
控制	CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

应用
应用	This Anti-Calcium Channel Antibody, Voltage Gated α 1A is validated for use in IC, IP, WB for the detection of Calcium Channel.
主要应用	Western Blotting Immunocytochemistry
应用说明	All procedures that are going to receive a full-length protein should be performed at 4 °C, and the following protease inhibitor mixture should be used: pepstatin A (1 mg/mL), leupeptin (1 mg/mL), aprotinin (1 mg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 mg/mL each). Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat brain sections.Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled second antibody. Western blot: 1.5 μg/mL (1:200) using ECL Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Purified peptide CNA1 from 865-881 of alpha 1A subunit of rat brain voltage-gated calcium channel (Starr, 1991) (Accession P54282) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Westenbroek et al. 1995).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Cav2.1 (alpha 1A subunit, P/Q-type of voltage-gated calcium channel, Cacna1a). Does not cross react with any other calcium channel proteins tested so far.
品种反应性	RatMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_023035.2 NM_000068.3
Entrez基因汇总	Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants have been described, however, the full-length nature of not all is known. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-16 to 21-28 in the coding region is associated with spinocerebellar ataxia 6.
基因符号	EA2 CACNA1A FHM CAV2.1 CACNL1A4 SCA6 BI HPCA CACN3 MHP1 Cav2.1 CACH4 APCA MHP
UniProt编号	O00555
UniProt汇总	FUNCTION: SwissProt: O00555 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the high-voltage activated (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin- IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA). SIZE: 2505 amino acids; 282365 Da SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. DOMAIN: SwissProt: O00555 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. DISEASE: SwissProt: O00555 # Defects in CACNA1A are the cause of spinocerebellar ataxia 6 (SCA6) [MIM:183086]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA6 is caused by expansion of a CAG repeat in the coding region of CACNA1A. There seems to be a correlation between the repeat number and earlier onset of the disorder. & Defects in CACNA1A are the cause of familial hemiplegic migraine (FHM) [MIM:141500]; also known as migraine familial hemiplegic 1 (MHP1). FHM, a rare autosomal dominant subtype of migraine with aura, is associated with ictal hemiparesis and, in some families, progressive cerebellar atrophy. & Defects in CACNA1A are the cause of episodic ataxia type 2 (EA-2) [MIM:108500]; also known as acetazolamide-responsive hereditary paroxysmal cerebellar ataxia (APCA). This autosomal dominant disorder is characterized by acetozolamide-responsive attacks of cerebellar ataxia and migraine-like symptoms, interictal nystagmus, and cerebellar atrophy. SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution add glycerol (ASC gade or better at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

包装信息
数量	200 µL

merck millipore,默克密理博,AB5152-200UL,Anti-Calcium Channel Antibody, Voltage Gated α 1A

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