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merck millipore,默克密理博,05-1322,Anti-Bmi-1 Antibody, clone DC9
产品名称：Anti-Bmi-1 Antibody, clone DC9
产品型号：05-1322
Use Anti-Bmi-1 Antibody, clone DC9 (Mouse Monoclonal Antibody) validated in WB, IP to detect Bmi-1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog.
merck millipore,默克密理博,05-1322,Anti-Bmi-1 Antibody, clone DC9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Ascites Monoclonal Antibody 描述 产品目录编号 05-1322 描述 Anti-Bmi-1 Antibody, clone DC9 Alternate Names - polycomb group ring finger 4
- B lymphoma Mo-MLV insertion region 1 homolog
- B lymphoma Mo-MLV insertion region 1 homolog (mouse)
- BMI1 polycomb ring finger oncogene
- Polycomb group RING finger protein 4
- RING finger protein 51
- murine leukemia viral (bmi-1) oncogene homolog
- oncogene BMI-1
背景信息 The Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer. 产品信息 格式 Ascites 控制 - U20S Cell Lysate
演示 Mouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-Bmi-1 Antibody, clone DC9 (Mouse Monoclonal Antibody) validated in WB, IP to detect Bmi-1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation1.生物信息 免疫原品种 Recombinant fusion protein of full length murine BMI-1 tagged with MBP. 克隆 DC9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes murine BMI-1 (~35 kDa). 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Human, Mouse. Not tested in other species.
抗体类型 Monoclonal Antibody Entrez基因编号 - NP_031578.2
基因符号 - flvi-2/bmi-1
- MGC12685
- PCGF4
- RNF51
纯化方法 Protein G purfied UniProt编号 - P35226
UniProt汇总 FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Ref.8 Ref.11
SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Ref.11 Ref.4 Ref.5 Ref.7 Ref.9
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Ref.11
PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
SIMILARITY: Contains 1 RING-type zinc finger.
产品使用声明 质量保证 Routinely evaluated by Western Blot on U20S cell lysate.
Western Blot Analysis:
1:5,000 of this lot detected BMI-1 on 15 μg/mL of U20S cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
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