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  • merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody

  • merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody

    产品名称：Anti-Vesicular GABA Transporter (VGAT) Antibody
    产品型号：AB5062P
    This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).

    merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Mk, R, M, HWB, ELISA, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5062P
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Vesicular GABA Transporter (VGAT) Antibody
    Alternate Names
    • Vesicular Inhibitory Amino Acid Transporter
    背景信息 Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
    产品信息
    格式 Affinity Purified
    控制
    • Brain tissue, rat retina lysate
    演示 Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA and 0.05% sodium azide.
    应用
    应用 This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).
    主要应用
    • Western Blotting
    • ELISA
    • Immunohistochemistry
    应用说明 Immunohistochemistry/Immunofluorescence:
    2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.

    ELISA:
    1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

    Western blot:
    1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
    表位 C-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
    品种反应性 MonkeyRatMouseHuman
    Species Reactivity Note Rat. Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_080552.2
    Entrez基因汇总 The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
    基因符号
    • hVIAAT
    • SLC32A1
    • VIAAT
    • VGAT
    • bA122O1.1
    纯化方法 Immunoaffinity purified
    UniProt编号
    • Q9H598
    UniProt汇总 FUNCTION: SwissProt: Q9H598 # Involved in the uptake of GABA and glycine into the synaptic vesicles.
    SIZE: 525 amino acids; 57415 Da
    SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
    TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
    SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family.
    产品使用声明
    质量保证 Evaluated by Western Blot on Rat retina lysates.

    Western Blot Analysis:
    1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody

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