- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB M Affinity Purified Monoclonal Antibody 描述 产品目录编号 05-1116 Replaces MAB1665 描述 Anti-VEGF Antibody, clone CH-10 Alternate Names - vascular endothelial growth factor isoform VEGF165
- Vascular permeability factor
- vascular endothelial growth factor
- vascular endothelial growth factor A
背景信息 VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. 产品信息 格式 Affinity Purified 控制 - Jurkat cell lysate
演示 Liquid in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6 containing 0.1% sodium azide. 应用 应用 Anti-VEGF Antibody, clone CH-10 is an antibody against VEGF for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant mouse VEGF. 克隆 CH-10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes human and rat VEGF, other variants not tested. 同种型 IgG1 品种反应性 RatHuman Species Reactivity Note Reacts with human and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_033531.3
Entrez基因汇总 This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. 基因符号 - VPF
- MGC70609
- MVCD1
- VEGF
- VEGF-A
纯化方法 Affinity Purfied UniProt编号 - P15692
UniProt汇总 FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to VEGFR2/Kdr but doesn't activate downstream signaling pathways, doesn't activate angiogenesis and inhibits tumor growth.
SUBUNIT STRUCTURE: Homodimer; disulfide-linked. Also found as heterodimer with PlGF By similarity.
SUBCELLULAR LOCATION: Secreted. Note: VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
INDUCTION: Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations.
SEQUENCE SIMILARITIES: Belongs to the PDGF/VEGF growth factor family.
SEQUENCE CAUTION: The sequence CAC19512.2 differs from that shown. Reason: Erroneous gene model prediction.产品使用声明 质量保证 Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 1:1000 dilution of this antibody detected VEGF on 10 µg of Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody
下一件merck millipore产品：merck millipore,默克密理博,4700-0060,Guava ViaCount Flex Reagent for Flow Cytometry
相关产品
merck millipore,默克密理博,MAB8261,Anti-Influenza A Antibody, H1N1, clone 9B3.2
merck millipore,默克密理博,MAB8251-KC,Mouse Anti-Influenza A (1mL) KC
merck millipore,默克密理博,04-323,Anti-AMPKα1 Antibody, rabbit monoclonal
merck millipore,默克密理博,69752,pET-24d(+) DNA - Novagen
merck millipore,默克密理博,AB5062P,Anti-Vesicular GABA Transporter (VGAT) Antibody
merck millipore,默克密理博,4700-0060,Guava ViaCount Flex Reagent for Flow Cytometry
merck millipore,默克密理博,MABF95,Anti-murine T cell receptor Vbeta 8.1 and 8.2 Antibody, clone KJ16-133
merck millipore,默克密理博,14-496,PKCβII Protein, active, 10 µg
merck millipore,默克密理博,04-077,Anti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonal
