merck millipore,默克密理博,AB4132,Anti-GATA4 Antibody

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merck millipore,默克密理博,AB4132,Anti-GATA4 Antibody
产品名称：Anti-GATA4 Antibody
产品型号：AB4132
Detect GATA4 with Anti-GATA4 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ICC & WB.

merck millipore,默克密理博,AB4132,Anti-GATA4 Antibody

产品介绍

merck millipore,默克密理博,AB4132,Anti-GATA4 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB4132
Replaces	MABE477
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-GATA4 Antibody
背景信息	GATA4 (GATA binding factor-4) is a member of the GATA family of transcription factors. GATA4 is involved in embryogenesis and in myocardial differentiation and function and is associated with heart septal defects.

产品信息
格式	Affinity Purified
控制	Heart tissue
演示	Affinity Purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Detect GATA4 with Anti-GATA4 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ICC & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Western Blot and Immunocytochemistry: 1/200 to 1/1000. Optimal dilutions must be determined by the user.

生物信息
免疫原品种	Synthetic peptide corresponding to amino acids 313-331 of human GATA4 (RKRKPKNLNKSKTP).
宿主	Rabbit
特异性	Recognizes GATA4.
品种反应性	RatHumanMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002052.2
Entrez基因汇总	This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
基因符号	MGC126629 GATA4
纯化方法	Immunoaffinity purified
UniProt编号	P43694
UniProt汇总	FUNCTION: SwissProt: P43694 # Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). SIZE: 442 amino acids; 44627 Da SUBUNIT: Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF (By similarity). SUBCELLULAR LOCATION: Nucleus. DISEASE: SwissProt: P43694 # Defects in GATA4 are a cause of atrial septal defect 2 (ASD2) [MIM:607941]. ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or non-cardiac abnormalities. SIMILARITY: SwissProt: P43694 ## Contains 2 GATA-type zinc fingers.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3–7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4–25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

储存和货运信息

存储条件

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3–7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4–25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.