merck millipore,默克密理博,AB4124,Anti-FOXA1 Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,AB4124,Anti-FOXA1 Antibody
产品名称：Anti-FOXA1 Antibody
产品型号：AB4124
Anti-FOXA1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXA1 also known as Forkhead Box Protein A1, Hepatocyte Nuclear Factor 3-alpha, HNF3A & has been validated in ICC.

merck millipore,默克密理博,AB4124,Anti-FOXA1 Antibody

产品介绍

merck millipore,默克密理博,AB4124,Anti-FOXA1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB4124
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-FOXA1 Antibody
Alternate Names	HNF3A Forkhead Box Protein A1 Hepatocyte Nuclear Factor 3-alpha

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-FOXA1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXA1 also known as Forkhead Box Protein A1, Hepatocyte Nuclear Factor 3-alpha, HNF3A & has been validated in ICC.
主要应用	Immunocytochemistry
应用说明	Immunocytochemistry: 1/200 to 1/1000. Optimal dilutions must be determined by the user.

生物信息
免疫原品种	Synthetic peptide corresponding to amino acids 388-399 of human FOXA1 (LKGDPHYSFNHP).
宿主	Rabbit
特异性	Recognizes FOXA1. The calculated molecular weight is ~49.1 kDa.
品种反应性	RatHumanMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_004496.2
Entrez基因汇总	This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
基因符号	HNF-3A FOXA1 HNF3A MGC33105 TCF3A
UniProt编号	P55317
UniProt汇总	FUNCTION: SwissProt: P55317 # Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. SIZE: 473 amino acids; 49321 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P55317 ## Contains 1 fork-head DNA-binding domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

储存和货运信息

存储条件

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.