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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA M Serum Monoclonal Antibody 描述 产品目录编号 MAB1810 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-ADAMTS-1 Antibody, clone 5D4E11B6 Alternate Names - METH-1
背景信息 ADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein. 产品信息 格式 Serum 控制 - MCF-7 cell lysate
- A431 cell lysate
- HEK293 cell lysate
- PC-12 cell lysate
- Embryonic mouse brain lysate
演示 Ascites. Liquid containing 0.05% NaN3. 应用 应用 Detect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1:2000 - 1:4000 dilution on A431 cell lysate
Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.
Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate生物信息 免疫原品种 Myc-His carboxyl tagged full-length recombinant protein based on the human sequence. 表位 Unknown 克隆 5D4E11B6 宿主 Mouse 特异性 Clone 5D4E11B6 recognizes human ADAMTS-1. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Reactivity with other species has not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006988.3
Entrez基因汇总 This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. 基因符号 - C3-C5
- ADAMTS1
- KIAA1346
- METH1
UniProt编号 - Q9UHI8
UniProt汇总 FUNCTION: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
CATALYTIC ACTIVITY: Cleaves aggrecan at the 1938-Glu-|-Leu-1939 site, within the chondroitin sulfate attachment domain.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
DOMAIN: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain and contains 1 peptidase M12B domain and contains 3 TSP type-1 domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C for up to 1 year from date of receipt. 包装信息 数量 100 µL
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