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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, ELISA, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB3505 Replaces AB3243 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-PDX-1 Antibody Alternate Names - Pancreas Duodenum Homeobox-1
产品信息 格式 Serum 控制 - POSITIVE CONTROL: Pancreas.
演示 Rabbit serum. Liquid. Contains no preservative. 应用 应用 Use Anti-PDX-1 Antibody (Rabbit Polyclonal Antibody) validated in ELISA, IP, WB, IHC to detect PDX-1 also known as Pancreas Duodenum Homeobox-1. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Western blot: 1:1,000-1:2,000 using ECL. The antibody reacts a protein of 30-40 kDa. Suggested blocking buffer is TBS containing 5% non-fat milk. Suggested gel percentage is 9%.
Immunohistochemistry: 1:200-1:500 on frozen and paraffin embedded tissues sections fixed with 4% paraformaldehyde. Suggested permeablization solution is alcohol. Blocking buffer should contain 5% nonfat milk and 5% horse serum. Suggested antigen retrieval solution is citrate buffer.
Immunoprecipitation: 5 μL/100 μL. Final reaction volume: 105 μL. Final total protein concentration in reaction mix: 1 mg/mL. Suggested capture agent is Protein A beads.
ELISA, indirect: 1:36,000Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the c-terminus of mouse PDX-1. 宿主 Rabbit 特异性 PDX-1 (Pancreas duodenum homeobox-1). The antibody reacts with both natural and recombinant protein. Epitope location is cytoplasmic and nuclear. 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000209.2
Entrez基因汇总 Insulin promoter factor 1 is an islet-specific protein that activates transcription of the insulin andsomatostatin genes. It is a key regulator of islet peptide hormone expressionn and also plays an essential role in pancreatic development. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus. 基因符号 - IUF-1
- PDX1
- IPF1
- IDX-1
- GSF
- IUF1
- IPF-1
- STF-1
- MODY4
- PDX-1
UniProt编号 - P52945
UniProt汇总 FUNCTION: SwissProt: P52945 # Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
SIZE: 283 amino acids; 30771 Da
SUBUNIT: Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP (By similarity).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
DOMAIN: SwissProt: P52945 The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter (By similarity). & The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein- protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y) (By similarity).
PTM: Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
DISEASE: SwissProt: P52945 # Defects in PDX1 are a cause of pancreatic agenesis [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant. & Defects in PDX1 are the cause of maturity onset diabetes noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type II. & Defects in PDX1 are the cause of maturity onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY [MIM:606391] is a form of diabetes mellitus characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion.
SIMILARITY: Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. & Contains 1 homeobox DNA-binding domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,6630,Uracil - CAS 66-22-8 - Calbiochem
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