merck millipore,默克密理博,AB3209,Anti-Oct-4 Antibody

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merck millipore,默克密理博,AB3209,Anti-Oct-4 Antibody
产品名称：Anti-Oct-4 Antibody
产品型号：AB3209
This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.

merck millipore,默克密理博,AB3209,Anti-Oct-4 Antibody

产品介绍

merck millipore,默克密理博,AB3209,Anti-Oct-4 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, ICC, IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB3209
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Oct-4 Antibody
Alternate Names	POU5F1
背景信息	Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

产品信息
格式	Affinity Purified
控制	Pluripotent human embryonic stem (ES) cells
演示	Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet. Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide, amino acids 282-297of Oct4.
宿主	Rabbit
特异性	Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_203289.3 NM_002701.4
基因符号	OTF3 POU5F1 Oct-3 OTF4 OCT3 OCT4 Oct-4 MGC22487 Oct4
纯化方法	Immunoaffinity purified
UniProt编号	Q01860
UniProt汇总	FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene. SIZE: 360 amino acids; 38571 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels. SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain. MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.