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品种反应性 主要应用 宿主 格式 抗体类型 R, M, H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB2201 描述 Anti-mPER1 (residues 6-21) Antibody Alternate Names - period homolog 1 (Drosophila)
- Circadian clock protein PERIOD 1
- Circadian pacemaker protein Rigui
- Period, drosophila, homolog of period 1
- period (Drosophila) homolog 1
- homolog of period 1
- period (Drosophila) homolog 1
背景信息 This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described. 产品信息 格式 Serum 控制 - Human brain lysate
演示 Serum with 0.05% NaN3 应用 应用 Detect mPER1 (residues 6-21) using this Anti-mPER1 (residues 6-21) Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Immunohistichemistry:
Tested on whole brain (SCN; peak staining at CT/ZT12). Optimium working dilutions need to be determined by end used.生物信息 免疫原品种 Recombinant Protein 表位 N-Terminus 宿主 Rabbit 特异性 Cat. # AB2201 will recognize the N-terminus of mPER1. 品种反应性 RatMouseHuman Species Reactivity Note Mouse, Human and Rat. May react with Drosophila, Arvicanthis niloticus and Mesocricetus auratus based on sequence homology. Reactivity with other species has not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - BAA94086
基因符号 - hPER1
- CAB2
- KIAA0482
- MGC88021
- MGC9753
- PER
- PERLD1
- RIGUI
- hPER
纯化方法 Unpurified UniProt编号 - O35973
UniProt汇总 FUNCTION: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences CLOCK function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation.
SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity).
INTERACTION:
Q9R1X4:Timeless; NbExp=2; IntAct=EBI-1266764, EBI-1793117;
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
TISSUE SPECIFICITY: In brain, highest expression is observed in the SCN. Highly expressed in the pyramidal cell layer of the piriform cortex, the periventricular part of the caudate-putamen, many thalamic nuclei, and the granular layer of the cerebellar cortex. Weaker expression is detected in most area of the brain, including cortical and non cortical structures. Expression but no oscillations occurs in the glomerular and mitral cell layers of the olfactory bulb, the internal granular layer of the cerebellum, the cornu ammonis and dentate gyrus of the hyppocampus, the cerebral and piriform cortices. Also found in heart, brain, spleen, lung, liver, skeletal muscle, testis. Highest level in testis. Low level in kidney.
DEVELOPMENTAL STAGE: Expressed in the suprachiasmatic nucleus (SCN) during late fetal and early neonatal life.
INDUCTION: In the suprachiasmatic nucleus (SCN), behaves like a day-type oscillator, with maximum expression during the light period. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. In liver, peak levels at CT9. In the SCN, levels increase by light exposure during subjective night. Circadian oscillations also observed in skeletal muscle and liver but not in testis.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation.
PTM: Ubiquitinated.
SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain.
SIMILARITY: Contains 2 PAS (PER-ARNT-SIM) domains.
产品使用声明 质量保证 Routinely tested by western blot on human brain lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. 包装信息 数量 100 µL
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