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merck millipore,默克密理博,05-1000,Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
产品名称:Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
产品型号:05-1000
Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
merck millipore,默克密理博,05-1000,Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt WB, ELISA, FC M Purified Monoclonal Antibody 描述 产品目录编号 05-1000 品牌系列 Upstate
商名 - Upstate
描述 Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) 背景信息 The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%). 产品信息 格式 Purified 控制 - Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
演示 Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol. 应用 应用 Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB. 主要应用 - Western Blotting
- ELISA
- Flow Cytometry
应用说明 Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA生物信息 免疫原品种 Phosphoserine coupled to KLH. 克隆 4A4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes serine-phosphorylated proteins from all species. 同种型 IgG1 品种反应性 Vertebrates Species Reactivity Note Reacts with vertebrates 抗体类型 Monoclonal Antibody 修改 - Phosphorylation
纯化方法 Protein G-Sepharose™ chromatography 产品使用声明 质量保证 Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.包装信息 数量 100 µg
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