merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1928,Anti-Integrin α5 Antibody, CT, Intracellular

  • merck millipore,默克密理博,AB1928,Anti-Integrin α5 Antibody, CT, Intracellular

    产品名称：Anti-Integrin α5 Antibody, CT, Intracellular
    产品型号：AB1928
    Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.

    merck millipore,默克密理博,AB1928,Anti-Integrin α5 Antibody, CT, Intracellular

  • 产品介绍
  • merck millipore,默克密理博,AB1928,Anti-Integrin α5 Antibody, CT, Intracellular

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, Ch, H, M, HtWB, ELISA, ICC, IHCRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB1928
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Integrin α5 Antibody, CT, Intracellular
    Alternate Names
    • Fibronectin Receptor alpha Subunit
    • CD49e
    背景信息 Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
    产品信息
    格式 Serum
    控制
    • Mouse 3T3 fibroblasts Skin (Basement Membrane).
    演示 Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
    应用
    应用 Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Western Blot Analysis:
    1:1000 dilution of a previous lot was used.

    ELISA:
    1:500-1:2000 dilution of a previous lot was used in ELISA.

    Immunoprecipitation:
    Recommended use of 5 μL of antibody for 5x106 cells.

    Immunohistochemistry:
    1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.

    Immunocytochemistry:
    1:1000 of a previous lot was used in immunocytochemistry.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.
    表位 C-terminus, intracellular
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
    品种反应性 RatChickenHumanMouseHamster
    Species Reactivity Note Human, mouse, rat, hamster, and chicken. Reactivity with other species has not been determined.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002196
    Entrez基因汇总 The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq]
    基因符号
    • FNRA
    • ITGA5
    • VLA-5
    • CD49e
    • VLA5A
    纯化方法 Unpurified
    UniProt编号
    • P08648
    UniProt汇总 FUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    SIZE: 1049 amino acids; 114536 Da
    SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
    SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
    SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on HUVEC lysates.

    Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

    Handling Recommendations:
    Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB1928,Anti-Integrin α5 Antibody, CT, Intracellular

上一件merck millipore产品：merck millipore,默克密理博,IL003,Interleukin-3 Protein, Recombinant human
下一件merck millipore产品：merck millipore,默克密理博,ABN261,Anti-POU6F1 Antibody


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