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品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB19032 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-ADAM 12 Antibody, CT Alternate Names - Meltrin alpha
产品信息 格式 Serum 演示 Neat rabbit serum. Contains no preservative. 应用 应用 Detect ADAM 12 using this Anti-ADAM 12 Antibody, C-terminus validated for use in WB. 主要应用 - Western Blotting
不建议的应用 - Flow Cytometry
应用说明 Western blot: 1:500-1:2000; purified milk proteins or BSA (2-3%) for blocking as milk can contain ADAMs and lower sensitivity. Do not dilute the primary antibody in milk; Simple TBS or PBS is recommended. Variable size on gel expected, as there are several processed forms of the protein. ~116-96kDa proforms, with 68kDa active forms predominately.
IP: not tested
FACS: Not suitable
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 13 amino acid synthetic peptide from the C-terminous of the protein (HQVPRPSHNAYIK) 表位 C-terminus 宿主 Rabbit 特异性 ADAM 12, cross reactivity with other ADAM family members untested. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_021641.2
- NM_003474.3
Entrez基因汇总 This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. 基因符号 - EC 3.4.24.-
- ADAM12
- Meltrin-alpha
- MLTN
- MCMP
- MLTNA
- Mltna
- OTTHUMP00000046766
- MCMPMltna
UniProt编号 - O43184
UniProt汇总 FUNCTION: SwissProt: O43184 # Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
COFACTOR: Binds 1 zinc ion per subunit.
SIZE: 909 amino acids; 99641 Da
SUBUNIT: Interacts with alpha-actinin-2 and with syndecans (By similarity). Interacts with SH3PXD2A.
SUBCELLULAR LOCATION: Isoform 12L: Cell membrane; Single-pass type I membrane protein. & Isoform 12S: Secreted.
TISSUE SPECIFICITY: Isoform 12L is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 12S seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
DOMAIN: SwissProt: O43184 The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading. & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 6 months. 包装信息 数量 100 µL
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