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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, IHC Sh Purified Polyclonal Antibody 描述 产品目录编号 AB1584 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-c-Fos Antibody Alternate Names - G0/G1 Switch Regulatory Protein 7
背景信息 c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states. 产品信息 格式 Purified 控制 - Widely expressed
演示 Purified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL). 应用 应用 Use Anti-c-Fos Antibody (Sheep Polyclonal Antibody) validated in IHC, IHC(P), WB to detect c-Fos also known as G0/G1 Switch Regulatory Protein 7. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry: 1:500-1:4000, 2-8°C 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.
Western blot: 10 μg/mL. Detects a band at approximately 55 kDa.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic c-fos peptide (residues Ser.Gly.Phe.Asn.Ala.Asp.Tyr.Glu.Ala. Ser.Ser.Ser.Arg.Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Sheep 特异性 Recognizes c-fos. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005252.2
Entrez基因汇总 The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. 基因符号 - c-fos
- FOS
- G0S7
纯化方法 Ammonium sulfate precipitation UniProt编号 - P01100
UniProt汇总 FUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
SIZE: 380 amino acids; 40695 Da
SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity).
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to one year.
Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.包装信息 数量 500 µg
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