merck millipore,默克密理博,AB1541,Anti-Tryptophan Hydroxylase Antibody

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merck millipore,默克密理博,AB1541,Anti-Tryptophan Hydroxylase Antibody
产品名称：Anti-Tryptophan Hydroxylase Antibody
产品型号：AB1541
Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.

merck millipore,默克密理博,AB1541,Anti-Tryptophan Hydroxylase Antibody

产品介绍

merck millipore,默克密理博,AB1541,Anti-Tryptophan Hydroxylase Antibody

Replacement Information
Replacement Information	AB1541 is a recommended replacement for AB1556

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	WB, IHC	Sh	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB1541
Replaces	AB1556
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Tryptophan Hydroxylase Antibody
Alternate Names	tryptophan hydroxylase 1 Tryptophan 5-monooxygenase 1 indoleacetic acid-5-hydroxylase tryptophan 5-hydroxylase tryptophan 5-monooxygenase tryptophan hydroxylase (tryptophan 5-monooxygenase)
背景信息	Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.

产品信息
格式	Affinity Purified
控制	PC12 lysates Brain tissue
演示	Purified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol.

应用
应用	Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry(peroxidase): Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Sheep
特异性	Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.
品种反应性	RatHuman
Species Reactivity Note	Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_004179.1
Entrez基因汇总	This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the gut, pineal gland, spleen, and thymus.
基因符号	EC 1.14.16.4 TPH1 TPRH MGC119994 TRPH TPH
纯化方法	Immunoaffinity purified
UniProt编号	P17752
UniProt汇总	COFACTOR:Fe(2+) ion. SIZE:444 amino acids; 50985 Da SUBUNIT:Multimer of identical subunits. TISSUE SPECIFICITY:Isoform 2 seems to be less widely expressed than isoform 1. SIMILARITY:SwissProt: P17752 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. & Contains 1 ACT domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.