merck millipore,默克密理博,AB143,Anti-Choline Acetyltransferase (ChAT) Antibody

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merck millipore,默克密理博,AB143,Anti-Choline Acetyltransferase (ChAT) Antibody
产品名称：Anti-Choline Acetyltransferase (ChAT) Antibody
产品型号：AB143
Anti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB.

merck millipore,默克密理博,AB143,Anti-Choline Acetyltransferase (ChAT) Antibody

产品介绍

merck millipore,默克密理博,AB143,Anti-Choline Acetyltransferase (ChAT) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Mk, H, R, M, Bat, Fe	WB, IHC	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	AB143
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Choline Acetyltransferase (ChAT) Antibody
Alternate Names	choline acetyltransferase Choline acetylase acetyl CoA:choline O-acetyltransferase
背景信息	Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

产品信息
格式	Serum
控制	Cerebral cortex tissue.
演示	Rabbit polyclonal antisera, liquid, no preservatives.

应用
应用	Anti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunoprecipitation: 1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals. Optimal working dilutions and protocls must be determined by end user.

生物信息
免疫原品种	Human placental enzyme.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Cholinergic neurons in brain and central nervous systems.
品种反应性	MonkeyHumanRatMouseBatFeline
Species Reactivity Note	Human, Mouse, Rat, Monkey, Bat, Feline. Reactivity with other species has not been confirmed with IHC.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_065574
Entrez基因汇总	This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]. Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.
基因符号	CMS1A2 CHAT ChAT CHOACTase CMS1A
纯化方法	Unpurified
UniProt编号	P28329
UniProt汇总	FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.

产品使用声明
质量保证	Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue. Immunohistochemistry (Paraffin) Analysis: ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis:
ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.